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THE BETTS LAB

Early Embryo and Pluripotent Stem Cell Laboratory

Our research program is aimed towards understanding the cellular and molecular pathways that regulate preimplantation embryo development and pluripotent stem cell function.  A main focus of the lab is to train the next generation of scientists, biotechnologists and entrepreneurs.

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ABOUT THE BETTS LAB

The Betts Lab utilizes unique cell and animal models including cutting-edge molecular cell biology techniques to study preimplantation embryos, stem cell function and cellular reprogramming events during early embryonic development and during the generation of induced pluripotent stem cells.

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Stem Cells, Inked Episode 5 (Metabolism & Cell Fate)

Stem Cells, Inked Episode 5 (Metabolism & Cell Fate)

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CURENT PROJECTS

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THE P66SHC ADAPTOR PROTEIN MAINTAINS PLURIPOTENCY AND REGULATES CELL FATE DURING EMBRYO DEVELOPMENT

CIHR submitted (PI: Betts)

The P66Shc adaptor protein belongs to the Src homology 2 domain containing (Shc) family of adaptor proteins that mediates cellular response to oxidative stress by translocating into the mitochondrial intermembrane space where it regulates metabolism and ROS production. Using established embryo-derived stem cell populations and early mouse embryo models, we will define the metabolic functions of p66Shc in regulating stem cell behavior (state) and p66Shc’s capacity to regulate stem cell differentiation into specialized cell types (fate).

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REDOX REGULATION OF CANINE PLURIPOTENT STEM CELLS

NSERC Discovery (PI: Betts)

These studies will identify redox-modified proteins that will begin to elucidate the redox signaling pathways responsible for regulating self-renewal and pluripotency and will enhance our understanding of the molecular mechanisms that regulate the transition from inner cell mass (ICM) to epiblast cell fate. Modulation of the redox state during the cellular reprogramming procedure will define specific mechanisms underlying redox regulation of pluripotency induction.

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EXTRA-TELOMERIC FUNCTIONS OF TELOMERASE REVERSE TRANSCRIPTASE (TERT) ISOFORMS

CIHR Operating (PI: Betts)

Expression of specific TERT isoforms likely promotes stem cell function via extra-telomeric means. These studies will elucidate the extra-telomeric roles for TERT isoforms and define new mechanisms controlling pluripotent stem cell function. These studies may reveal novel therapeutic options to enhance tissue regeneration, differentiation and repair and to treat age-related diseases such as cancer.

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NON-INVASIVE IDENTIFICATION OF CONDITIONED CULTURE MEDIA CONSTITUENTS TO DETERMINE EMBRYO DEVELOPMENTAL COMPETENCE

CHRI & OB/GYN TRGF (PI: Basim Rafea)

The proposed studies are directed at characterizing microRNAs and mRNAs that are released into culture medium by preimplantation embryos. We expect to define patterns of microRNA and mRNAs that are indicative of normal preimplantation development and allow for a molecular indication of embryonic developmental competence post embryo transfer.

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UNDERSTANDING AND TREATING OBESITY RELATED LOSS OF EMBRYO DEVELOPMENTAL COMPETENCE

CIHR submitted (PI: Watson)

We will investigate the possible ways in which obesity may restrict early mouse and human development by treating early mouse and human embryos with non-esterified fatty acids (NEFAs) that are sharply elevated in the serum and ovarian fluids of obese patients. We will also investigate ways in which early embryos attempt to avoid and adapt to maternal obesity effects imposed on their development. Our experiments will advance effective embryo culture medium development and will initiate approaches that could result in the production of therapeutic culture medium. Our ultimate goal is to improve the developmental capacity of early embryos and the success of assisted reproductive technologies.

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SOLE FUEL SOURCE SELECTION STRATEGY TO ENHANCE PLURIPOTENCY

OIRM New Ideas Grant (PIs: Betts and Cumming)

In this project we will direct fibroblasts towards a single and/or bivalent metabolic states using sole fuel source selection to determine if their induction towards multiple pluripotent states is enhanced. Pluripotency will be assessed using various molecular markers of general, naïve, and primed pluripotency, while interrogation of metabolic pathways will be carried out by metabolic measurements and assessing various markers of glycolysis and OXPHOS. These studies will develop a simple and reliable culture protocol to modulate the bioenergetic state of somatic cells to efficiently generate high quality naïve human iPSCs for future stem cell therapies.

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LAB MEMBERS

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DEAN H. BETTS, PHD

Professor

BSc Honours Cell Biology, Western
MSc Zoology, Western
PhD Biomedical Sciences, Guelph
PDF Genetics, CWRU

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QUINN

Executive Assistant

PhD Best Doggo, Bett's School

Project: The lab's lab

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ZULEIKA C. L. LEUNG

Research Assistant

BMSc Interdisciplinary Medical Sciences, Western 

MSc Physiology and Pharmacology & Developmental Biology, Western 

Project: The impact of free fatty acids on preimplantation embryo autophagy

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ALEX KOZLOV

PhD Candidate (Co-supervisor Robert Cumming)


BSc Honors Biology, Western

Project: Examining the relationship between lactate and cell fate.

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DAVID HAWKE

PhD Candidate

BSc Chemical Engineering, Queen's

MASc Chemical Engineering, Queen's

Project: Exosome-derived miRNAs as  non-invasive predictors of preimplantation embryo competence.

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ANDREW POWELL

PhD Candidate (Co-supervisor Robert Cumming)

BASc Engineering Chemistry, Queen's

MASc Chemical Engineering, Queen's

Project: The role of p66Shc in neural stem cell maintenance and differentiation.


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VIRGINIA WOLFE

MSc Candidate

BSc Honours Physiology, McMaster University

Project: The role of p66Shc in regulating preimplantation embryo metabolism

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STEFAN GRAHOVAC

MSc Candidate

BSc Honours Specialization Biology, Western

Project: Genome-wide CRISPR-Cas9 screen to discover novel functional interactions of the p66Shc adaptor protein in regulating pluripotency

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WINNY WANG

Admin & Outreach, MA Student

BSc Biology and Political Science, Western

FEATURED PUBLICATION

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SMALL-MOLECULE INDUCTION OF CANINE EMBRYONIC STEM CELLS TOWARD NAÏVE PLURIPOTENCY

Tobias et al. Stem Cells Dev. 2016 Aug 15;25(16):1208-22.

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PUBLICATIONS

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EVALUATING AUTOPHAGY IN PREIMPLANTATION EMBRYOS

July 21, 2022

Leung ZCL, Hunter H, Abu Rafea B, Watson AJ, and Betts DH (2022). Evaluating Autophagy in Preimplantation Embryos. Autophagy Reports 1(1): 309-337.

MODULATION OF PKM1/2 LEVELS BY STERIC BLOCKING MORPHOLINOS ALTERS THE METABOLIC AND PLURIPOTENT STATE OF MURINE PLURIPOTENT STEM CELLS

June 8, 2022

Dierolf JG, Hunter HLM, Watson AJ, Betts DH (2022). Modulation of PKM1/2 levels by steric blocking morpholinos alters the metabolic and pluripotent state of murine pluripotent stem cells. Stem Cells and Development 31 (11-12): 278-295.

FREE FATTY ACID TREATMENT OF MOUSE PREIMPLANTATION EMBRYOS DEMONSTRATES CONTRASTING EFFECTS OF PALMITIC ACID AND OLEIC ACID ON AUTOPHAGY

May 1, 2022

Leung ZCL, Calder MD, Abu-Rafea B, Watson AJ, Betts DH (2022). Free fatty acid treatment of mouse preimplantation embryos demonstrates contrasting effects of palmitic acid and oleic acid on autophagy. Am J Physiol Cell Physiol. 322(5): C833-C848.

THE EFFECTS OF OBESITY AND NON-ESTERIFIED FATTY ACIDS ON PREIMPLANTATION EMBRYO DEVELOPMENT

2022

Leung ZCL, Betts DH, Watson AJ (2022). The effects of obesity and non-esterified fatty acids on preimplantation embryo development. Trends Dev Biol 14: 19-31.  

3D IMMUNOFLUORESCENT IMAGE COLOCALIZATION QUANTIFICATION IN MOUSE EPIBLAST STEM CELLS

April 29, 2022

Dierolf JG, Watson AJ, Betts DH (2022). 3D immunofluorescent image colocalization quantification in mouse epiblast stem cells. Methods in Molecular Biology 2490: 69-79. 

FLOW CYTOMETRIC CHARACTERIZATION OF PLURIPOTENT CELL PROTEIN MARKERS IN NAÏVE, FORMATIVE, AND PRIMED PLURIPOTENT STEM CELLS

April 29, 2022

Dierolf JG, Chadwick K, Brooks CR, Watson AJ, Betts DH (2022). Flow Cytometric Characterization of Pluripotent Cell Protein Markers in Naïve, Formative, and Primed Pluripotent Stem Cells. Methods in Molecular Biology 2490: 81-92. 

EFFECTS OF PALMITIC ACID ON LOCALIZATION OF EMBRYO CELL FATE AND BLASTOCYST FORMATION GENE PRODUCTS

February 14, 2022

Calder MD, Chen R, MacDonald A, MacNeily Z, Leung ZCL, Adus A, Cui S, Betts DH, Abu-Rafea B, and Watson AJ (2022). Effects of palmitic acid on localization of embryo cell fate and blastocyst formation gene products. Reproduction 163(3): 133-143. 

CELL THERAPY IN VETERINARY MEDICINE AS A PROOF-OF CONCEPT FOR HUMAN THERAPIES: PERSPECTIVES FROM THE NORTH AMERICAN VETERINARY MEDICINE ASSOCIATION

November 30, 2021

Arzi B, Webb TL, Koch TG, Volk SW, Betts DH, Watts A, Goodrich L, Kallos MS, Kol A (2021). Cell Therapy in Veterinary medicine as a Proof-of Concept for Human Therapies: Perspectives from the North American Veterinary Medicine Association. Front. Vet. Sci. doi: 10.3389/fvets.2021.779109.

DIFFERENTIAL LOCALIZATION PATTERNS OF PYRUVATE KINASE ISOFORMS IN MURINE NAÏVE, FORMATIVE AND PRIMED PLURIPOTENT STATES

June 26, 2021

Dierolf JG, Watson AJ, Betts DH (2021). Differential localization patterns of pyruvate kinase isoforms in murine naïve, formative and primed pluripotent states. Experimental Cell Research 405(2):112714.

MURINE BLASTOCYSTS RELEASE MATURE MICRORNAS INTO CULTURE MEDIA THAT REFLECT DEVELOPMENTAL STATUS

May 28, 2021

Hawke DC, Watson AJ, Betts DH (2021). Murine blastocysts release mature microRNAs into culture media that reflect developmental status. Frontiers in Genetics 12:655882. 

ANALYSIS OF TERT ISOFORMS ACROSS TCGA, GTEX AND CCLE DATASETS

April 13, 2021

Subasri M, Shooshtari P, Watson AJ, Betts DH (2021). Analysis of TERT Isoforms Across TCGA, GTEx and CCLE Datasets. Cancers 13(8):1853. 

PANNEXIN 1 BINDS Β-CATENIN TO MODULATE MELANOMA CELL GROWTH AND METABOLISM

February 26, 2021

Sayedyahossein S, Huang K, Li Z, Zhang C, Kozlov AM, Johnston D, Nouri-Nejad D, Dagnino L, Betts DH, Sacks DB and Penuela S (2021). Pannexin 1 binds β-catenin to modulate melanoma cell growth and metabolism. J Biol Chem 296:100478. 

EXTRACELLULAR VESICLES, MICRORNA AND THE PREIMPLANTATION EMBRYO: NON-INVASIVE CLUES OF EMBRYO WELL-BEING

January 1, 2021

Hawke DC, Watson AJ, Betts DH (2021). Extracellular Vesicles, MicroRNA and the Preimplantation Embryo: Non-Invasive Clues of Embryo Well-Being. Reproductive BioMedicine Online 41(1): 39-54. 

TARGETED EXPRESSION PROFILING REVEALS DISTINCT STAGES OF EARLY CANINE FIBROBLAST REPROGRAMMING ARE REGULATED BY 2-OXOGLUTARATE HYDROXYLASES

December 9, 2020

Tobias ICKao MC, Parmentier T, Hunter H, LaMarre J and Betts DH (2020). Targeted expression profiling reveals distinct stages of early canine fibroblast reprogramming are regulated by 2-oxoglutarate hydroxylases. Stem Cell Res Ther 11(1):528. 

THE USE OF INDUCED PLURIPOTENT STEM CELLS IN DOMESTIC ANIMALS: A NARRATIVE REVIEW

December 8, 2020

Scarfone RA, Pena SM, Russell KA, Betts DH and Koch TG (2020). The Use of Induced Pluripotent Stem Cells in Domestic Animals: A Narrative Review. BMC Veterinary Research 16(1):477.

OLEIC ACID COUNTERS IMPAIRED BLASTOCYST DEVELOPMENT INDUCED BY PALMITIC ACID DURING MOUSE PREIMPLANTATION DEVELOPMENT: UNDERSTANDING OBESITY RELATED DECLINES IN FERTILITY

June 10, 2020

Yousif MD, Calder MD, Du JT, Ruetz KN, Crocker K, Urquhart BL, Betts DH, Rafea BA, Watson AJ (2020). Oleic Acid Counters Impaired Blastocyst Development Induced by Palmitic Acid during Mouse Preimplantation Development: Understanding Obesity Related Declines in Fertility. Reproductive Sciences 27(11):2038-2051.

LACTATE PRECONDITIONING PROMOTES A HIF-1-ALPHA-MEDIATED METABOLIC SHIFT FROM OXPHOS TO GLYCOLYSIS IN NORMAL HUMAN DIPLOID FIBROBLASTS

May 20, 2020

Kozlov AM, Lone A, Betts DH*, Cumming RC* (2020). Lactate preconditioning promotes a HIF-1-alpha-mediated metabolic shift from OXPHOS to glycolysis in normal human diploid fibroblasts. Scientific Reports 10: 8388. *Co-senior authors. 

DYNAMIC REGULATION OF CONNEXINS IN STEM CELL PLURIPOTENCY

October 28, 2019

Esseltine JL, Brooks C, Edwards NA, Subasri M, Sampson J, Seguin C, Betts DH and Laird DW (2020). Dynamic regulation of connexins in stem cell pluripotency. Stem Cells 38(1):52-66. 

TRAPPING AND SERS IDENTIFICATION OF EXTRACELLULAR VESICLES USING NANOHOLE ARRAYS.

March 7, 2019

Kaufman LH, Cooper T, Wallace GQ, Hawke DC, Betts D, Hess D, Lagugné-Labarthet F (2019). Trapping and SERS identification of extracellular vesicles using nanohole arrays. Proc. SPIE 10894, Plasmonics in Biology and Medicine XVI, 108940B.

P66SHC ACTIVATION PROMOTES INCREASED OXIDATIVE PHOSPHORYLATION AND RENDERS CNS CELLS MORE VULNERABLE TO AMYLOID BETA TOXICITY

November 20, 2018

Lone A, Harris R, Singh O, Betts DH, Cumming RC. (2018). p66Shc activation promotes increased oxidative phosphorylation and renders CNS cells more vulnerable to amyloid beta toxicity. Scientific Reports 8(1):17081. 

LOSS OF P66SHC PROMOTES PRIMITIVE ENDODERM IDENTITY IN THE INNER CELL MASS OF MOUSE BLASTOCYSTS

November 1, 2018

Edwards NA, Watson AJ, Betts DH (2018). Loss of p66Shc promotes primitive endoderm identity in the inner cell mass of mouse blastocysts. Stem Cells and Development 27(21):1479-1493.

ANALYSIS OF MITOCHONDRIAL DIMENSIONS AND CRYSTAL STRUCTURE IN PLURIPOTENT STEM CELLS USING TRANSMISSION ELECTRON MICROSCOPY

November 2018

Tobias IC, Khazaee RBetts DH (2018). Analysis of mitochondrial dimensions and crystal structure in pluripotent stem cells using transmission electron microscopy. Current Protocols in Stem Cell Biology 47(1):e67. 

https://currentprotocols.onlinelibrary.wiley.com/doi/abs/10.1002/cpsc.67

METABOLIC PLASTICITY DURING TRANSITION TO NAÏVE-LIKE PLURIPOTENCY IN CANINE EMBRYO-DERIVED STEM CELLS

May 17, 2018

Tobias IC, Isaac RR, Dierolf J, Khazaee R, Cumming RC and Betts DH (2018). Metabolic plasticity during transition to naïve-like pluripotency in canine embryo-derived stem cells. Stem Cell Research 30:22-33. 

https://www.sciencedirect.com/science/article/pii/S1873506118301235?via%3Dihub

CD-1 MOUSE FERTILITY RAPIDLY DECLINES AND IS ACCOMPANIED WITH EARLY PREGNANCY LOSS UNDER CONVENTIONAL HOUSING CONDITIONS

March 1, 2018

Crocker K, Calder MD, Edwards NA, Betts DH, Watson AJ. CD-1 mouse fertility rapidly declines and is accompanied with early pregnancy loss under conventional housing conditions. Theriogenology. 2018 Mar 1;108:245-254.

TREATMENT WITH AICAR INHIBITS BLASTOCYST DEVELOPMENT, TROPHECTODERM DIFFERENTIATION AND TIGHT JUNCTION FORMATION AND FUNCTION IN MICE

November 1, 2017

Calder MD, Edwards NA, Betts DH, Watson AJ. Treatment with AICAR inhibits blastocyst development, trophectoderm differentiation and tight junction formation and function in mice. Mol Hum Reprod. 2017 Nov 1;23(11):771-785.

CONNEXIN43 MUTANT PATIENT-DERIVED INDUCED PLURIPOTENT STEM CELLS EXHIBIT ALTERED DIFFERENTIATION POTENTIAL

June 2016

Esseltine JL, Shao Q, Brooks C, Sampson J, Betts DH, Séguin CA, Laird DW. Connexin43 Mutant Patient-Derived Induced Pluripotent Stem Cells Exhibit Altered Differentiation Potential. J Bone Miner Res. 2017 Jun;32(6):1368-1385.

CHARACTERIZATION AND IMMUNOMODULATORY EFFECTS OF CANINE ADIPOSE TISSUE- AND BONE MARROW-DERIVED MESENCHYMAL STROMAL CELLS

December 1, 2016

Russell KA, Chow NH, Dukoff D, Gibson TW, LaMarre J, Betts DH, Koch TG. Characterization and Immunomodulatory Effects of Canine Adipose Tissue- and Bone Marrow-Derived Mesenchymal Stromal Cells. PLoS One. 2016 Dec 1;11(12):e0167442.

SMALL MOLECULE INDUCTION OF CANINE EMBRYONIC STEM CELLS TOWARDS NAIVE PLURIPOTENCY

August 15, 2016

Tobias IC, Brooks CR, Teichroeb JH, Villagómez DA, Hess DA, Séguin CA, Betts DH. Small-Molecule Induction of Canine Embryonic Stem Cells Toward Naïve Pluripotency. Stem Cells Dev. 2016 Aug 15;25(16):1208-22.

P66SHC, A KEY REGULATOR OF METABOLISM AND MITOCHONDRIAL ROS PRODUCTION, IS DYSREGULATED BY MOUSE EMBRYO CULTURE

September 2016

Edwards NA, Watson AJ, Betts DH. P66Shc, a key regulator of metabolism and mitochondrial ROS production, is dysregulated by mouse embryo culture. Mol Hum Reprod. 2016 Sep;22(9):634-47.

THE ROLE OF TELOMERES AND TELOMERASE REVERSE TRANSCRIPTASE ISOFORMS IN PLURIPOTENCY INDUCTION AND MAINTENANCE

August 2, 2016

Teichroeb JH, Kim J, Betts DH. The role of telomeres and telomerase reverse transcriptase isoforms in pluripotency induction and maintenance. RNA Biol. 2016 Aug 2;13(8):707-19.

CANINE PLURIPOTENT STEM CELLS: ARE THEY READY FOR CLINICAL APPLICATIONS?

October 7, 2015

Betts DH, Tobias IC. Canine Pluripotent Stem Cells: Are They Ready for Clinical Applications? Front Vet Sci. 2015 Oct 7;2:41.

DELIVERING ANTISENSE MORPHOLINO OLIGONUCLEOTIDES TO TARGET TELOMERASE SPLICE VARIANTS IN HUMAN EMBRYONIC STEM CELLS

2016

Radan L, Hughes CS, Teichroeb JH, Postovit LM, Betts DH. Delivering Antisense Morpholino Oligonucleotides to Target Telomerase Splice Variants in Human Embryonic Stem Cells. Methods Mol Biol. 2016;1341:133-42.

MICROENVIRONMENTAL REGULATION OF TELOMERASE ISOFORMS IN HUMAN EMBRYONIC STEM CELLS

September 1, 2014

Radan L, Hughes CS, Teichroeb JH, Vieira Zamora FM, Jewer M, Postovit LM, Betts DH. Microenvironmental regulation of telomerase isoforms in human embryonic stem cells. Stem Cells Dev. 2014 Sep 1;23(17):2046-66.

THE P66(SHC) ADAPTOR PROTEIN CONTROLS OXIDATIVE STRESS RESPONSE IN EARLY BOVINE EMBRYOS

January 24, 2014

Betts DH, Bain NT, Madan P. The p66(Shc) adaptor protein controls oxidative stress response in early bovine embryos. PLoS One. 2014 Jan 24;9(1):e86978.

DERIVATION AND CULTURE OF CANINE EMBRYONIC STEM CELLS

2013

Tobias IC, Brooks CR, Teichroeb JH, Betts DH. Derivation and culture of canine embryonic stem cells. Methods Mol Biol. 2013;1074:69-83.

EARLY EMBRYONIC DEVELOPMENT, ASSISTED REPRODUCTIVE TECHNOLOGIES, AND PLURIPOTENT STEM CELL BIOLOGY IN DOMESTIC MAMMALS

August 2013

Hall V, Hinrichs K, Lazzari G, Betts DH, Hyttel P. Early embryonic development, assisted reproductive technologies, and pluripotent stem cell biology in domestic mammals. Vet J. 2013 Aug;197(2):128-42.

STRESS-INDUCIBLE PHOSPHOPROTEIN 1 HAS UNIQUE COCHAPERONE ACTIVITY DURING DEVELOPMENT AND REGULATES CELLULAR RESPONSE TO ISCHEMIA VIA THE PRION PROTEIN

September 2013

Beraldo FH, Soares IN, Goncalves DF, Fan J, Thomas AA, Santos TG, Mohammad AH, Roffé M, Calder MD, Nikolova S, Hajj GN, Guimaraes AL, Massensini AR, Welch I, Betts DH, Gros R, Drangova M, Watson AJ, Bartha R, Prado VF, Martins VR, Prado MA. Stress-inducible phosphoprotein 1 has unique cochaperone activity during development and regulates cellular response to ischemia via the prion protein. FASEB J. 2013 Sep;27(9):3594-607.

ELEVATED P66SHC IS ASSOCIATED WITH INTRACELLULAR REDOX IMBALANCE IN DEVELOPMENTALLY COMPROMISED BOVINE EMBRYOS

January 2013

Bain NT, Madan P, Betts DH. Elevated p66Shc is associated with intracellular redox imbalance in developmentally compromised bovine embryos. Mol Reprod Dev. 2013 Jan;80(1):22-34.

THE LONG AND SHORT OF IT: THE ROLE OF TELOMERES IN FETAL ORIGINS OF ADULT DISEASE

October 3, 2012

Hallows SE, Regnault TR, Betts DH. The long and short of it: the role of telomeres in fetal origins of adult disease. J Pregnancy. 2012;2012:638476.

MASS SPECTROMETRY-BASED PROTEOMIC ANALYSIS OF THE MATRIX MICROENVIRONMENT IN PLURIPOTENT STEM CELL CULTURE

December 2012

Hughes C, Radan L, Chang WY, Stanford WL, Betts DH, Postovit LM, Lajoie GA. Mass spectrometry-based proteomic analysis of the matrix microenvironment in pluripotent stem cell culture. Mol Cell Proteomics. 2012 Dec;11(12):1924-36.

LONG TELOMERES BYPASS THE REQUIREMENT FOR TELOMERE MAINTENANCE IN HUMAN TUMORIGENESIS

February 23, 2012

Taboski MA, Sealey DC, Dorrens J, Tayade C, Betts DH*, Harrington L*. Long telomeres bypass the requirement for telomere maintenance in human tumorigenesis. Cell Rep. 2012 Feb 23;1(2):91-8. *Co-senior authors.

LOW LEVELS OF X-INACTIVE SPECIFIC TRANSCRIPT IN SOMATIC CELL NUCLEAR TRANSFER EMBRYOS DERIVED FROM FEMALE BOVINE FREEMARTIN DONOR CELLS

2012

Jeon BG, Rho GJ, Betts DH, Petrik JJ, Favetta LA, King WA. Low levels of X-inactive specific transcript in somatic cell nuclear transfer embryos derived from female bovine freemartin donor cells. Sex Dev. 2012;6(1-3):151-9.

SUPPRESSION OF THE IMPRINTED GENE NNAT AND X-CHROMOSOME GENE ACTIVATION IN ISOGENIC HUMAN IPS CELLS

October 2011

Teichroeb JH, Betts DH, Vaziri H. Suppression of the imprinted gene NNAT and X-chromosome gene activation in isogenic human iPS cells. PLoS One. 2011;6(10):e23436.

PROTEOMIC ANALYSIS OF EXTRACELLULAR MATRICES USED IN STEM CELL CULTURE

October 2011

Hughes CS, Radan L, Betts D, Postovit LM, Lajoie GA. Proteomic analysis of extracellular matrices used in stem cell culture. Proteomics. 2011 Oct;11(20):3983-91.

OSTEOGENIC DIFFERENTIATION OF EQUINE CORD BLOOD MULTIPOTENT MESENCHYMAL STROMAL CELLS WITHIN CORALLINE HYDROXYAPATITE SCAFFOLDS IN VITRO

2011

Figueroa RJ, Koch TG, Betts DH. Osteogenic differentiation of equine cord blood multipotent mesenchymal stromal cells within coralline hydroxyapatite scaffolds in vitro. Vet Comp Orthop Traumatol. 2011;24(5):354-62.

IN VITRO AND IN VIVO GERM LINE POTENTIAL OF STEM CELLS DERIVED FROM NEWBORN MOUSE SKIN

2011

Dyce PW, Liu J, Tayade C, Kidder GM, Betts DH, Li J. In vitro and in vivo germ line potential of stem cells derived from newborn mouse skin. PLoS One. 2011;6(5):e20339.

SYNAPTICALLY-COMPETENT NEURONS DERIVED FROM CANINE EMBRYONIC STEM CELLS BY LINEAGE SELECTION WITH EGF AND NOGGIN.

2011

Wilcox JT, Lai JK, Semple E, Brisson BA, Gartley C, Armstrong JN, Betts DH. Synaptically-competent neurons derived from canine embryonic stem cells by lineage selection with EGF and Noggin. PLoS One. 2011;6(5):e19768.

THE EARLY EMBRYO RESPONSE TO INTRACELLULAR REACTIVE OXYGEN SPECIES IS DEVELOPMENTALLY REGULATED

2011

Bain NT, Madan P, Betts DH. The early embryo response to intracellular reactive oxygen species is developmentally regulated. Reprod Fertil Dev. 2011;23(4):561-75.

IN VITRO DEVELOPMENTAL POTENTIAL OF NUCLEAR TRANSFER EMBRYOS CLONED WITH ENUCLEATION METHODS USING PRE-DENUDED BOVINE OOCYTES

December 2011

Jeon BG, Betts DH, King WA, Rho GJ. In vitro developmental potential of nuclear transfer embryos cloned with enucleation methods using pre-denuded bovine oocytes. Reprod Domest Anim. 2011 Dec;46(6):1035-42.

STEM CELL ROLES IN REPRODUCTION: WHAT IS THE BASIC SCIENCE?

November 2010

Betts DH, Kalionis B, Hillier SG. Stem cell roles in reproduction: what is the basic science? Mol Hum Reprod. 2010 Nov;16(11):791-2.

VIABLE IPSC MICE: A STEP CLOSER TO THERAPEUTIC APPLICATIONS IN HUMANS?

February 2010

Betts DH, Kalionis B. Viable iPSC mice: a step closer to therapeutic applications in humans? Mol Hum Reprod. 2010 Feb;16(2):57-62.

IMPROVED ISOLATION PROTOCOL FOR EQUINE CORD BLOOD-DERIVED MESENCHYMAL STROMAL CELLS

2009

Koch TG, Thomsen PD, Betts DH. Improved isolation protocol for equine cord blood-derived mesenchymal stromal cells. Cytotherapy. 2009;11(4):443-7.

CURRENT AND FUTURE REGENERATIVE MEDICINE - PRINCIPLES, CONCEPTS, AND THERAPEUTIC USE OF STEM CELL THERAPY AND TISSUE ENGINEERING IN EQUINE MEDICINE

February 2009

Koch TG, Berg LC, Betts DH. Current and future regenerative medicine - principles, concepts, and therapeutic use of stem cell therapy and tissue engineering in equine medicine. Can Vet J. 2009 Feb;50(2):155-65.

CHARACTERIZATION OF CANINE EMBRYONIC STEM CELL LINES DERIVED FROM DIFFERENT NICHE MICROENVIRONMENTS

October 2009

Wilcox JT, Semple E, Gartley C, Brisson BA, Perrault SD, Villagómez DA, Tayade C, Becker S, Lanza R, Betts DH. Characterization of canine embryonic stem cell lines derived from different niche microenvironments. Stem Cells Dev. 2009 Oct;18(8):1167-78.

CONCEPTS FOR THE CLINICAL USE OF STEM CELLS IN EQUINE MEDICINE

October 2008

Koch TG, Berg LC, Betts DH. Concepts for the clinical use of stem cells in equine medicine. Can Vet J. 2008 Oct;49(10):1009-17.

PERMANENT EMBRYO ARREST: MOLECULAR AND CELLULAR CONCEPTS

August 2008

Betts DH, Madan P. Permanent embryo arrest: molecular and cellular concepts. Mol Hum Reprod. 2008 Aug;14(8):445-53.

S-ADENOSYLHOMOCYSTEINE TREATMENT OF ADULT FEMALE FIBROBLASTS ALTERS X-CHROMOSOME INACTIVATION AND IMPROVES IN VITRO EMBRYO DEVELOPMENT AFTER SOMATIC CELL NUCLEAR TRANSFER

June 2008

Jeon BG, Coppola G, Perrault SD, Rho GJ, Betts DH, King WA. S-adenosylhomocysteine treatment of adult female fibroblasts alters X-chromosome inactivation and improves in vitro embryo development after somatic cell nuclear transfer. Reproduction. 2008 Jun;135(6):815-28.

ALTERNATIVE SPLICING AND EXPRESSION ANALYSIS OF BOVINE DNA METHYLTRANSFERASE 1

April 2008

Russell DF, Betts DH. Alternative splicing and expression analysis of bovine DNA methyltransferase 1. Dev Dyn. 2008 Apr;237(4):1051-9.

THE OXIDATIVE STRESS ADAPTOR P66SHC IS REQUIRED FOR PERMANENT EMBRYO ARREST IN VITRO

November 29, 2007

Favetta LA, Madan P, Mastromonaco GF, St John EJ, King WA, Betts DH. The oxidative stress adaptor p66Shc is required for permanent embryo arrest in vitro. BMC Dev Biol. 2007 Nov 29;7:132.

EARLY PREGNANCY DIAGNOSIS BY SERUM PROGESTERONE AND ULTRASOUND IN SHEEP CARRYING SOMATIC CELL NUCLEAR TRANSFER-DERIVED PREGNANCIES

April 2008

Alexander B, Coppola G, Mastromonaco GF, St John E, Reyes ER, Betts DH, King WA. Early pregnancy diagnosis by serum progesterone and ultrasound in sheep carrying somatic cell nuclear transfer-derived pregnancies. Reprod Domest Anim. 2008 Apr;43(2):207-11.

STEM CELL THERAPY FOR JOINT PROBLEMS USING THE HORSE AS A CLINICALLY RELEVANT ANIMAL MODEL

November 2007

Koch TG, Betts DH. Stem cell therapy for joint problems using the horse as a clinically relevant animal model. Expert Opin Biol Ther. 2007 Nov;7(11):1621-6.

LOW OXYGEN DELAYS FIBROBLAST SENESCENCE DESPITE SHORTER TELOMERES

February 2008

Betts DH, Perrault SD, King WA. Low oxygen delays fibroblast senescence despite shorter telomeres. Biogerontology. 2008 Feb;9(1):19-31.

ISOLATION OF MESENCHYMAL STEM CELLS FROM EQUINE UMBILICAL CORD BLOOD

May 30, 200

Koch TG, Heerkens T, Thomsen PD, Betts DH. Isolation of mesenchymal stem cells from equine umbilical cord blood. BMC Biotechnol. 2007 May 30;7:26.

TELOMERE LENGTH STATUS OF SOMATIC CELL SHEEP CLONES AND THEIR OFFSPRING

December 2007

Alexander B, Coppola G, Perrault SD, Peura TT, Betts DH, King WA. Telomere length status of somatic cell sheep clones and their offspring. Mol Reprod Dev. 2007 Dec;74(12):1525-37.

USE OF SOMATIC CELL NUCLEAR TRANSFER TO STUDY MEIOSIS IN FEMALE CATTLE CARRYING A SEX-DEPENDENT FERTILITY-IMPAIRING X-CHROMOSOME ABNORMALITY

Spring 2007

Rho GJ, Coppola G, Sosnowski J, Kasimanickam R, Johnson WH, Semple E, Mastromonaco GF, Betts DH, Koch TG, Weese S, Hewson J, Hayes MA, Kenney DG, Basrur PK, King WA. Use of somatic cell nuclear transfer to study meiosis in female cattle carrying a sex-dependent fertility-impairing X-chromosome abnormality. Cloning Stem Cells. 2007 Spring;9(1):118-29.

HIGH LEVELS OF P66SHC AND INTRACELLULAR ROS IN PERMANENTLY ARRESTED EARLY EMBRYOS

April 15, 2007

Favetta LA, St John EJ, King WA, Betts DH. High levels of p66shc and intracellular ROS in permanently arrested early embryos. Free Radic Biol Med. 2007 Apr 15;42(8):1201-10.

GENOMIC STABILITY AND PHYSIOLOGICAL ASSESSMENTS OF LIVE OFFSPRING SIRED BY A BULL CLONE, STARBUCK II

January 1, 2007

Ortegon H, Betts DH, Lin L, Coppola G, Perrault SD, Blondin P, King WA. Genomic stability and physiological assessments of live offspring sired by a bull clone, Starbuck II. Theriogenology. 2007 Jan 1;67(1):116-26.

ROLE OF CHROMOSOME STABILITY AND TELOMERE LENGTH IN THE PRODUCTION OF VIABLE CELL LINES FOR SOMATIC CELL NUCLEAR TRANSFER

August 9, 2006

Mastromonaco GF, Perrault SD, Betts DH, King WA. Role of chromosome stability and telomere length in the production of viable cell lines for somatic cell nuclear transfer. BMC Dev Biol. 2006 Aug 9;6:41.

THE IMPACT OF OOCYTE MATURATION MEDIA ON EARLY BOVINE EMBRYONIC DEVELOPMENT

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